Accurate quantitation for in vitro refolding of single domain antibody fragments expressed as inclusion bodies by referring the concomitant expression of a soluble form in the periplasms of Escherichia coli

详细信息    查看全文

• 作者：Tomoaki Noguchi ; Yuichi Nishida ; Keiji Takizawa ; Yue Cui ; Koki Tsutsumi ; Takashi Hamada ; Yoshisuke Nishi ; ^{y_nishi@nagahama-i-bio.ac.jp}
• 关键词：CD ; Circular Dichroism ; ELISA ; enzyme-linked immunosorbent assay ; TCDR ; three-step cold dialysis refolding ; OHDR ; one-step hot dialysis refolding ; OCDR ; one-step cold dialysis refolding ; CDR ; complementarity determining region ; HV ; hypervariable region ; CBB ; Coomassie Brilliant Blue ; IPTG ; isopropyl β-D-1-thiogalactopyranoside ; SDS ; sodium dodecyl sulfate ; PAGE ; polyacrylamide gel electrophoresis ; Gdn-HCl ; guanidinium chloride ; DTT ; dithiothreitol ; 2-ME ; 2-mercaptoethanol ; EDTA ; ethylenediaminet
• 刊名：Journal of Immunological Methods
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：442
• 期：Complete
• 页码：1-11
• 全文大小：711 K
• 卷排序：442

文摘

Precisely quantitative evaluation of refolded single chain antibodies from a cytoplasmic insoluble fraction was provided.