Liujunzi Tang, a famous traditional Chinese medicine, ameliorates cigarette smoke-induced mouse model of COPD

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• 作者：Rui Zhou^a ; ¹ ; Fen Luo^a ; ¹ ; Hui Lei^b ; Kai Zhang^c ; Jingyan Liu^a ; He He^a ; Jin Gao^a ; Xiayun Chang^a ; Ling He^d ; Hui Ji^d ; Tianhua Yan^a ; ^{yantianhuabest@126.com" class="auth_mail" title="E-mail the corresponding author} ; Tong Chen^a ; ^d ; ^{tongchencpu@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Liujunzi Tang ; Cigarette Smoke ; Anti-oxidation ; NF-κB
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：193
• 期：Complete
• 页码：643-651
• 全文大小：2023 K

文摘

Liujunzi Tang is a traditional herbal medicine widely used in East Asia and clinically applied to treat Phlegm-Heat Syndrome. The purpose of the present study was to investigate the protective effects of Liujunzi Tang on cigarette smoke-induced (CS) mouse model of chronic obstructive pulmonary disease (COPD) and explore its potential molecular mechanism.

Materials and methods

The mice received 1 h of cigarette smoke for 8 weeks. The serum levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 were determined by enzyme-linked immunosorbent assay (ELISA) kits. Superoxide dismutase (SOD) and malondialdehyde (MDA) were tested by biochemical methods. Histopathological alteration was observed by hematoxylin–eosin (H&E) staining. Additionally, the expressions of nuclear transcription factor-κB (NF-κBp65) and (inhibitor of NF-κB)IκB-α were determined by western blot and immunohistochemistry analysis.

Results

Liujunzi Tang enhanced the activities of antioxidant enzymes and attenuated the levels of lipid oxidative production, meanwhile significantly inhibited the generations of inflammatory cytokines by inhibiting the phosphorylation of IκB-α and NF-κB.

Conclusion

Our findings indicated that Liujunzi Tang exhibited the protective effect on cigarette smoke-induced COPD mice by anti-inflammatory and anti-oxidative properties through the inhibition of NF-κB activation.