Silk based bioinks for soft tissue reconstruction using 3-dimensional (3D) printing with in vitro and in vivo assessments

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• 作者：Marí ; a J. Rodriguez^a ; Joseph Brown^a ; Jodie Giordano^a ; Samuel J. Lin^b ; Fiorenzo G. Omenetto^a ; David L. Kaplan^a ; ^{david.kaplan@tufts.edu}
• 关键词：Soft tissue ; Bioink ; 3D printing ; Silk ; Regeneration
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：117
• 期：Complete
• 页码：105-115
• 全文大小：2002 K
• 卷排序：117

文摘

In the field of soft tissue reconstruction, custom implants could address the need for materials that can fill complex geometries. Our aim was to develop a material system with optimal rheology for material extrusion, that can be processed in physiological and non-toxic conditions and provide structural support for soft tissue reconstruction. To meet this need we developed silk based bioinks using gelatin as a bulking agent and glycerol as a non-toxic additive to induce physical crosslinking. We developed these inks optimizing printing efficacy and resolution for patient-specific geometries that can be used for soft tissue reconstruction. We demonstrated in vitro that the material was stable under physiological conditions and could be tuned to match soft tissue mechanical properties. We demonstrated in vivo that the material was biocompatible and could be tuned to maintain shape and volume up to three months while promoting cellular infiltration and tissue integration.