Methylated N-(4-N,N-dimethylaminocinnamyl) chitosan-coated electrospray OVA-loaded microparticles for oral vaccination

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• 作者：Tittaya Suksamran^a ; Tanasait Ngawhirunpat^a ; Theerasak Rojanarata^a ; Warayuth Sajomsang^b ; Tasana Pitaksuteepong^c ; Praneet Opanasopit^a ; ^{opraneet@hotmail.com} ; ^{praneet@su.ac.th}
• 关键词：Alginate ; Chitosan derivatives ; Microparticles ; Oral vaccine delivery ; Ovalbumin
• 刊名：International Journal of Pharmaceutics
• 出版年：2013
• 出版时间：1 May, 2013
• 年：2013
• 卷：448
• 期：1
• 页码：19-27
• 全文大小：1024 K

文摘

The purpose of this study was to prepare microparticles entrapping ovalbumin (OVA) as a model antigen to induce immune responses in mice following oral vaccination. In this study, calcium-alginate and calcium-yam-alginate microparticles were prepared by crosslinking alginate with calcium chloride solution using an electrospraying technique. 0.1 % (w/v) of methylated N-(4-N,N-dimethylaminocinnamyl) chitosan (TM₆₅CM₅₀CS) was used to coat microparticles entrapping an initial OVA of 20 % w/w to polymer. The results indicated that the coated microparticles were spherical and had a smooth surface, with an average size of 1-3 ¦Ìm, and were positively charged. In addition, the particles demonstrated a greater swelling and mucoadhesive properties than did uncoated microparticles. The in vitro release from the microparticles indicated that the coated microparticles resulted in more sustained release than uncoated microparticles. The cytotoxicity results showed that all of the formulations were safe. The in vivo oral administration demonstrated that at the same amount of 250 ¦Ìg OVA, coated microparticles exhibited the highest in vivo adjuvant activity in both IgG and IgA immunogenicity.