Differential methylation of the circular DNA in geminiviral minichromosomes
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- 作者：Kathrin Deuschle ; Gabi Kepp ; Holger Jeske ; holger.jeske@bio.uni-stuttgart.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Geminiviridae ; Begomovirus ; Abutilon mosaic virus ; tomato yellow leaf curl Sardinia virus ; Minichromosome purification ; DNA methylation ; Nicotiana benthamiana ; Ligation-mediated RCA
- 刊名：Virology
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：499
- 期：Complete
- 页码：243-258
- 全文大小：3313 K
文摘
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Separation of geminiviral minichromosomes according to nucleosome loading.
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Identification of methylated cccDNA in minichromosomes.
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Precise methylation loci determined.
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Deep sequencing of methylated loci using ligation-mediated rolling circle .amplification.