A new simply and effective fractionation method for cylindrospermopsin analyses

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• 作者：Kubo ; Takuya ; Sano ; Tomoharu ; Hosoya ; Ken ; Tanaka ; Nobuo ; Kaya ; Kunimitsu
• 关键词：CYN ; Fractionation ; Double-cartridges
• 刊名：Toxicon
• 出版年：2005
• 出版时间：July, 2005
• 年：2005
• 卷：46
• 期：1
• 页码：104-107
• 全文大小：213 K

文摘

A new simply and effective fractionation method for cylindrospermopsin (CYN) analyses was developed. The extract from cells of Cylindrospermopsis raciborskii was resuspended with 0.1 M carbonate buffer at pH 10.5, and pass through the double-cartridges column which was consisted of a styrene polymer cartridge and an anion exchange cartridge. CYN and deoxy-CYN were adsorbed with the anion exchange cartridge. After separation of the anion exchange cartridge, adsorbed compounds were eluted from the cartridge with 50 % methanol containing 1 % formic acid solution. CYN and deoxy-CYN were selectively condensed in the eluted solution. When CYN was analyzed by LC-photodiode array or LC/MS, only two peaks of CYN and deoxy-CYN were detected quantitatively. The results suggest that the fractionation method is a useful method for CYN analyses and must be utilized for CYN purification.