Evaluation of drug toxicity with hepatocytes cultured in a micro-space cell culture system

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• 作者：Kazuaki  ; Nakamura¹ ; Reiko  ; Mizutani¹ ; Atsushi  ; Sanbe¹ ; Shin  ; Enosawa² ; Mureo  ; Kasahara³ ; Atsuko  ; Nakagawa⁴ ; Yoko  ; Ejiri¹ ;   ; ⁵ ; Norie  ; Murayama⁶ ; Yuki  ; Miyamoto¹ ; Tomohiro  ; Torii¹ ; Shinji  ; Kusakawa¹ ; Junji  ; Yamauchi¹ ; Motohiro  ; Fukuda⁵ ; Hiroshi  ; Yamazaki⁶ ; Akito  ; Tanoue¹ ;   ; ^{atanoue@nch.go.jp}
• 关键词：HepG2 cells ; Hepatocyte ; Three-dimension culture ; Micro-space cell culture ; Hepatocyte-specific function ; Drug toxicity
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：111
• 期：1
• 页码：78-84
• 全文大小：1154 K

文摘

A micro-space cell culture system was recently developed in which cells such as hepatocytes can be cultured and formed into a multicellular three-dimensional (3D) architecture. In this study, we assessed the performance of HepG2 cells cultured in this micro-space cell culture system in a drug toxicity test, and evaluated the effects of micro-space culture on their hepatocyte-specific functions. The micro-space cell culture facilitated the formation of 3D HepG2 cell architecture. HepG2 cells cultured in a micro-space culture plate exhibited increased albumin secretion and enhanced mRNA expression levels of cytochrome P450 (CYP) enzyme compared to those cultured in a monolayer culture. When the cells were exposed to acetaminophen, a hepatotoxic drug, the damage to the HepG2 cells grown in micro-space culture was greater than the damage to the HepG2 cells grown in monolayer culture. In addition, human primary hepatocytes grown in micro-space culture also exhibited increased albumin secretion, enhanced CYP mRNA expression levels and increased sensitivity to acetaminophen compared to those grown in monolayer culture. These results suggest that this micro-space culture method enhances the hepatocyte-specific functions of hepatocytes, including drug-metabolizing enzyme activities, making hepatocytes grown in the micro-space culture system a useful tool for evaluating drug toxicity in vitro.