Efficiency of organic solvents on the ability of ¦Á2,3-sialyltransferase from Photobacterium sp. JT-ISH-224 to control a hydrolysis side reaction

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• 作者：Izuru Nagashima ; Toshiki Mine ; Takeshi Yamamoto ; Hiroki Shimizu
• 关键词：Enzymatic synthesis ; Sialyltransferase ; Sialidase activity ; Sialoside
• 刊名：Carbohydrate Research
• 出版年：2012
• 出版时间：1 September, 2012
• 年：2012
• 卷：358
• 期：Complete
• 页码：31-36
• 全文大小：364 K

文摘

Enzymatic synthesis of oligosaccharides using specific sialyltransferases enables single-step glycosylation with high positional and anomeric structural selectivity. The ¦Á2,3-sialyltransferase cloned from the marine bacterium Photobacterium sp. JT-ISH-224 has unique and broad acceptor specificity, but this enzyme possesses not only sialyltransferase activity but also sialidase activity. To synthesize sialoside derivative effectively, only sialyltransferase activity is required. We report here that addition of organic solvents was effective to control the sialidase activity and a resulting product was not hydrolyzed. The enzyme was even active in the presence of acetonitrile, ethanol, methanol, or acetone. To determine the suitable concentrations of these organic solvents, only sialyltransferase activity could be allowed, and as a result, the stable synthesis of sialoside could be achieved.