Use of PCR to estimate the prevalence of Equus caballus papillomavirus in aural plaques in horses

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• 作者：Ana Claudia Gorino ; Jose P. Oliveira-Filho ; Sueli A. Taniwaki ; Roberta M. Basso ; Luiza S. Zakia ; Jo?o P. Araujo Jr. ; Alex ; re S. Borges
• 关键词：Horse ; Aural plaques ; Papillomavirus ; PCR
• 刊名：The Veterinary Journal
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：197
• 期：3
• 页码：903-904
• 全文大小：215 K

文摘

Aural plaques occur on the skin of the medial surface of the pinnae of horses. In this study the presence of Equus caballus papillomavirus (EcPV)-3 and -4 DNA was assessed in 45 such plaques using a ¡®touchdown¡¯ PCR. Papillomaviruses (PVs) were detected in 62.3 % (28/45) of samples: EcPV-3 and -4 DNA in 8.89 % (4/45) and 37.78 % (17/45) of samples, respectively, with 15.56 % (7/45) of samples exhibiting co-infection. Viral DNA was not detected in 37.78 % (17/45) of samples, suggesting the possible existence of other equine PVs. Neither EcPV-3 nor -4 were detected in negative control skin. This study is the first to evaluate the prevalence of these two viruses in equine aural plaques.