Cryo-EM in the study of challenging systems: the human transcription pre-initiation complex

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• 作者：Eva Nogales¹ ; ² ; ³ ; ^{enogales@lbl.gov} ; Robert K Louder⁴ ; Yuan He⁵
• 刊名：Current Opinion in Structural Biology
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：40
• 期：Complete
• 页码：120-127
• 全文大小：2246 K
• 卷排序：40

文摘

Cryo-EM description of human TFIID conformational range and its link to DNA binding. Atomic modeling of TFIID DNA-binding subunits following masked refinement. Visualizing the stepwise assembly of human PIC led to robust subunit localization. High-resolution PIC structures in different states shed light on promoter opening.