Preferential expression of IGHV and IGHD encoding antibodies with exceptionally long CDR3H and a rapid global shift in transcriptome characterizes development of bovine neonatal immunity

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• 作者：Yfke Pasman^a ; Daniele Merico^b ; Azad K. Kaushik^a ; ^{akaushik@uoguelph.ca}
• 关键词：Next generation sequencing ; Neonate ; CDR3H ; Bovine ; Colostrum ; Immunoglobulin ; Antibody repertoire ; VDJ ; Cytokine ; Transcriptional factors ; Neonatal immunity
• 刊名：Developmental & Comparative Immunology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：67
• 期：Complete
• 页码：495-507
• 全文大小：1869 K
• 卷排序：67

文摘

A rapid global shift in bovine neonatal transcriptome occurs within first week post-birth that includes 110 differentially expressed immune-related genes. The IGHV1S1(BF4E9) and longest IGHD2(DH2) genes that encode bovine antibodies with exceptionally long CDR3H are preferentially expressed at birth indicating their important role in B development, selection and expansion. Unlike adult, IGKC is expressed at higher levels in the bovine neonates where IGKV12 is preferentially expressed at birth. Bovine neonate acquires innate and IgM-mediated humoral immunocompetence within first week of life.