The first report of mycovirus infection of the powdery mildew fungi.
Multiple dsRNA were detected from field-collected samples of powdery mildew fungi.
Deep sequencing of dsRNA revealed the presence of nine yeast and one smut fungal totivirus-like viruses.
Similar totvirus-like sequences are found in transcriptome shotgun assembly (TSA) libraries of the fungi, plants and insects.
These putative totiviruses and totvirus-like TSA sequences are phylogenetically clustered with members of the genus Totivirus, but not of the genus Victorivirus.