Characterization of human auditory brainstem circuits by calcium-binding protein immunohistochemistry
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- 作者：R.J. Kulesza Jr.
- 关键词：AN ; auditory nerve ; CB ; calbindin ; CB+ ; calbindin immunopositive ; CBP ; calcium-binding proteins ; CN ; cochlear nucleus ; CR ; calretinin ; CR+ ; calretinin immunopositive ; GBC ; globular bushy cell ; LNTB ; lateral nucleus of the trapezoid body ; LSO ; lateral superior olive ; MNTB ; medial nucleus of the trapezoid body ; MSO ; medial superior olive ; NDS ; normal donkey serum ; PB ; phosphate buffer ; PV ; parvalbumin ; SBC ; spherical bushy cell ; SOC ; superior olivary complex ; SPON ; superior paraolivary nucleus ; tz
- 刊名：Neuroscience
- 出版年：31 January, 2014
- 年：2014
- 卷：258
- 期：Complete
- 页码：318-331
- 全文大小：3479 K
文摘
The cochlear nucleus (CN) and superior olivary complex are auditory brainstem centers with essential roles in encoding temporal features of vocalizations, localization of sound sources and descending modulation of the cochlea. Numerous neuronal populations, across a multitude of laboratory mammals, have been characterized within these brainstem centers based on cell body morphology, dendritic architecture, afferent/efferent connections and neurochemistry. However, scant details are available for these neuronal populations in humans. The objective of this study is to further characterize human auditory hindbrain nuclei and examine the axonal connections between these structures. To this end, we have used immunohistochemistry and morphometric techniques to characterize neuronal populations and axonal projections in the human brainstem. Herein, we provide evidence for calretinin immunoreactive neurons and synaptic boutons in the ventral CN, axons in the trapezoid body, peridendritic boutons in the medial superior olive and calyceal endings in the medial nucleus of the trapezoid body (MNTB). Further, we demonstrate that the majority of neurons in the human MNTB are calbindin and Kv3.1b immunoreactive and that perisomatic profiles in this nucleus are vesicular glutamate transporter and Rab3a positive, suggesting that such profiles are in fact synaptic terminals.