Recent advances in solution NMR spectroscopy have led to new avenues for study, focusing on functional dynamics of molecular machines and characterization of rare conformational states of proteins.

Concrete examples of use of solution NMR spectroscopy to study high-molecular-mass systems and rare conformational states are described.

Atomic-resolution characterization of dynamic processes in biomolecules provided by NMR will be potentiated through complementary experimental studies involving X-ray crystallography, cryo-electron microscopy and computational studies.

Current challenges in the field are discussed.