Electrofiltration as a purification strategy for microbial poly-(3-hydroxybutyrate)

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• 作者：G?zde G?zke ; Carolin Prechtl ; Frank Kirschh?fer ; Gisela Mothes ; Jelka Ondruschka ; Gerald Brenner-Weiss ; Ursula Obst ; Clemens Posten
• 关键词：PHB ; poly-(3-hydroxybutyrate)
• 刊名：Bioresource Technology
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：123
• 期：Complete
• 页码：272-278
• 全文大小：632 K

文摘

The biodegradable polyester poly-(3-hydroxybutyrate) (PHB), produced by Ralstonia eutropha in batch and fed-batch processes, was purified by electrofiltration. The protein film on PHB granules determines their high negative zeta potential, enabling the application of electrofiltration as an integrated technology in the downstream processing of PHB. In order to determine the optimal purification parameters, various pressure and electric field strength conditions were tested. Electrofiltration of PHB at 4 bars and 4 V/mm provided an up to four times higher concentration factor than conventional filtration. FT-Raman spectroscopy demonstrated that electrofiltration did not result in structural changes to the products. The study demonstrates the efficiency and practical advantages of electrofiltration as a promising downstream step in the PHB production technology.