Kinetic resolution of racemic styrene oxide at a high concentration by recombinant Aspergillus usamii epoxide hydrolase in an n-hexanol/buffer biphasic system
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- 作者：Die Hua ; 1 ; Rui Wanga ; 1 ; Xiao-Ling Shib ; Hui-Hua Yeb ; Qin Wua ; Min-Chen Wuc ; biowmc@126.com" class="auth_mail" title="E-mail the corresponding author ; Jian-Jun Chud ; wschujianjun@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Kinetic resolution ; High substrate concentration ; Epoxide hydrolase ; Biphasic system ; (S)-Styrene oxide
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：152-158
- 全文大小：1613 K
文摘
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An n-hexanol/buffer biphasic system was built to improve biocatalytic efficiency.
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Kinetic resolution of 1 M rac-SO was successfully achieved by reAuEH2.
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(S)-SO was obtained in a 98.2% e.e. and a 34.3% yield within only 2 h.
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It showed high volumetric productivity in the gram-scale resolution of rac-SO.