In vitro study of the antibacterial properties and impact strength of dental acrylic resins modified with a nanomaterial

详细信息    查看全文

• 作者：Denise T. de Castro ; DDS<sup>asup> ; Mariana L.C. Valente ; DDS<sup>bsup> ; José ; Augusto M. Agnelli ; PhD<sup>csup> ; Clá ; udia H. Lovato da Silva ; PhD<sup>dsup> ; Evandro Watanabe ; PhD<sup>esup> ; Renato L. Siqueira ; DDS<sup>fsup> ; Oswaldo L. Alves ; PhD<sup>gsup> ; Raphael D. Holtz ; PhD<sup>hsup> ; André ; a C. dos Reis ; PhD<sup>isup><sup> ; sup><sup>andreare@forp.usp.br" class="auth_mail" title="E-mail the corresponding authorsup>
• 刊名：The Journal of Prosthetic Dentistry
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：115
• 期：2
• 页码：238-246
• 全文大小：4729 K

文摘

The accumulation of bacteria on the surface of dental prostheses can lead to systemic disease.

sSec_2">Purpose

spara0015">The purpose of this in vitro study was to evaluate the growth of Staphylococcus aureus and Pseudomonas aeruginosa on the surface of autopolymerizing (AP) and heat-polymerizing (HP) acrylic resins incorporated with nanostructured silver vanadate (β-AgVO<sub>3sub>) and its impact strength.

sSec_3">Material and methods

spara0020">For each resin, 216 circular specimens (9&times;2 mm) were prepared for microbiologic analysis and 60 rectangular specimens (65&times;10&times;3.3 mm) for mechanical analysis, according to the percentage of β-AgVO<sub>3sub>: 0%, control group; 0.5%; 1%; 2.5%; 5%; and 10%. After a biofilm had formed, the metabolic activity of the bacteria was measured using the XTT reduction assay (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) (n=8), and the number of viable cells was determined by counting colony forming units per milliliter (CFU/mL) (n=8). Confocal laser scanning microscopy (CLSM) was used to complement the analyses (n=2). The mechanical behavior was evaluated by impact strength assays (n=10). Data were analyzed by 2-way ANOVA, followed by the Tukey honestly significant difference (HSD) post hoc test (α=.05).

sSec_4">Results

spara0025">The addition of 5% and 10% β-AgVO<sub>3sub> significantly decreased the metabolic activity of P. aeruginosa for both resins (P<.05). The HP resin promoted a greater reduction in metabolic activity than the AP resin (P<.05). No difference was found in the metabolic activity of S. aureus according to the XTT (P>.05). The number of CFU/mL for S. aureus and P. aeruginosa decreased significantly when 5% and 10% β-AgVO<sub>3sub> were added (P<.001). These concentrations significantly reduced the impact strength of the resins (P<.001) because the system was weakened by the presence of clusters of β-AgVO<sub>3sub>.

sSec_5">Conclusion

spara0030">The addition of β-AgVO<sub>3sub> can provide acrylic resins with antibacterial activity but reduces their impact strength. More efficient addition methods should be investigated.