Evaluation of the correlation of caspofungin MICs and treatment outcome in murine infections by wild type strains of Candida parapsilosis

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• 作者：Valentina Salas ; F. Javier Pastor ; Javier Capilla ; Deanna A. Sutton ; Emilio Mayayo ; Annette W. Fothergill ; Michael G. Rinaldi ; Josep Guarro
• 关键词：Caspofungin ; Animal models ; Fungal infections ; C. parapsilosis
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：77
• 期：1
• 页码：41-45
• 全文大小：1176 K

文摘

We have evaluated the in vitro activity of caspofungin against 36 wild-type strains of Candida parapsilosis sensu stricto using 3 techniques: broth microdilution, disk diffusion, and the determination of minimal fungicidal concentration (MFC). The first 2 methods showed a good in vitro activity of caspofungin, but the MFCs were ¡Ý2 dilutions above their corresponding MICs. In a murine model of disseminated infection, we evaluated the efficacy of caspofungin at 5 mg/kg against 8 strains of C. parapsilosis representing different degrees of in vitro susceptibility (0.12-1 ¦Ìg/mL). All the isolates responded to treatment and (1¡ú3)-¦Â-D-glucan levels were reduced in all the cases; however, the study revealed differences among isolates, since caspofungin reduced the tissue burden of mice infected with isolates with MICs ¡Ü0.5 ¦Ìg/mL but was less effective against those with MICs of 1 ¦Ìg/mL.