Quantitative assay of element mass inventories in single cell biological systems with micro-PIXE

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• 作者：Nina Ogrinc^a ; ^e ; Primo? Pelicon^a ; ^{primoz.pelicon@ijs.si} ; Primo? Vavpeti?^a ; Mitja Kelemen^a ; Nata&scaron ; a Grlj^a ; Luka Jeromel^a ; Sergej Tomi?^b ; Miodrag ?oli?^b ; ^c ; Alfred Beran^d
• 关键词：Gold nanoparticles ; Tinntinopsis radix ; Zooplankton ; Lorica ; Element inventory ; Dendritic cells ; Particle induced X-ray emission-PIXE ; Elastic backscattering spectroscopy-EBS ; Scanning transmission ion microscopy-STM ; Focused proton beam ; Trace elements
• 刊名：Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
• 出版年：2013
• 出版时间：1 July, 2013
• 年：2013
• 卷：306
• 期：Complete
• 页码：121-124
• 全文大小：1124 K

文摘

Elemental concentrations in micro-PIXE (Particle Induced X-ray Emission) maps of elements in biological tissue slices have been determined using auxiliary information on the sample matrix composition from EBS (Elastic Backscattering Spectroscopy) and STIM (Scanning Transmission Ion Microscopy). The thin sample approximation may be used for evaluating micro-PIXE data in cases, where X-ray absorption in the sample can be neglected and the mass of elements in a selected area can be estimated. The resulting sensitivity amounts to an impressive 10^?12 g of the selected elements. Two cases are presented as examples. In the first, we determined the total mass of gold nanoparticles internalized by human monocyte-derived dendritic cells (MDDC). In the second, an inventory of the mass of elements in the micro-particulate material adsorbed at the wall of the lorica of the microzooplankton species Tintinnopsis radix has been created.