Molecular and enzymatic characterization of alkaline lipase from Bacillus amyloliquefaciens E1PA isolated from lipid-rich food waste
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- 作者：Thanakorn Saengsangaa ; Wilailak Siripornadulsila ; b ; Surasak Siripornadulsila ; b ; surasak@kku.ac.th" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bacillus amyloliquefaciens ; Alkaline lipase ; Pentapeptide ; Fatty acyl esters ; Lipid-rich food wastes ; Vegetable oils
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：82
- 期：Complete
- 页码：23-33
- 全文大小：2029 K
文摘
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Alkaline lipase of Bacillus amyloliquefaciens E1PA was successfully expressed and purified.
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E1PA lipase was moderately thermotolerant and stable over pH 4–10.
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E1PA lipase was able to hydrolyze a variety of substrates and heterogeneous lipid-rich food waste.
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Both intracellular and extracellular E1PA lipases were effective in lipid hydrolysis.
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Recombinant lipase showed potential for development for effective lipase production.