Construction of Nef-positive doxycycline-dependent HIV-1 variants using bicistronic expression elements
详细信息 查看全文
- 作者：Yme U. van der Velden1 ; Wendy Kleibeuker1 ; Alex Harwig ; Bep Klaver ; Esther Siteur-van Rijnstra ; Esmay Frankin ; Ben Berkhout ; Atze T. Das ; a.t.das@amc.uva.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：HIV ; Nef ; IRES ; 2A peptide ; Ubiquitin ; Tet-On system ; Protein production ; HIS mouse
- 刊名：Virology
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：488
- 期：Complete
- 页码：96-107
- 全文大小：778 K
文摘
- class="listitem" id="list_li0005">
- class="label">•
Different approaches to encode additional proteins in the HIV-1 genome were tested.
- class="label">•
IRES translation elements are incompatible with efficient HIV-1 replication.
- class="label">•
Ubiquitin and 2A fusion protein approaches allow efficient HIV-1 replication.
- class="label">•
Doxycycline-controlled HIV-1 variants that encode all viral proteins were developed.
- class="label">•
Nef stimulates HIV-rtTA replication in primary cells and human immune system mice.