Natural diterpene β-lactone derivative as photosystem II inhibitor on spinach chloroplasts

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• 作者：Beatriz King-Dí ; az ; Amé ; rica Pé ; rez-Reyes ; Flá ; vio José ; Leite dos Santos ; Dalton Luiz Ferreira-Alves ; Dorila Piló ; Veloso ; Salvador Uribe Carvajal and Blas Lotina-Hennsen
• 关键词：Diterpenes ; Lactones ; PSII electron inhibitors
• 刊名：Pesticide Biochemistry and Physiology
• 出版年：2006
• 出版时间：February 2006
• 年：2006
• 卷：84
• 期：2
• 页码：109-115
• 全文大小：156 K

文摘

α,7β-Dihydroxyvouacapan-17β-oic acid (1) was isolated from Pterodon polygalaeflorus Benth. Then, (1) was modified to obtain 6α-hydroxyvouacapan-7β,17β-lactone (2). Inhibition properties of both (1) and (2) were evaluated in spinach chloroplasts. Only compound (2) inhibited non-cyclic electron transport and ATP synthesis, i.e., (2) behaved as a Hill reaction inhibitor. Compound (2) did not affect photosystem I (PSI) activity but it did inhibit electron flow through PSII. Analysis of the partial PSII reactions from water to DCPIP_ox, water to silicomolybdate, and diphenylcarbazide to DCPIP_ox allowed locating inhibition sites at (i) the oxygen-evolving complex (OEC) and (ii) the redox enzymes of the electron transport chain, in the span of P₆₈₀ to Q_A. Chlorophyll a fluorescence measurements confirmed the action site for (2).