Epitope mapping and protective immunity elicited by adenovirus expressing the Leishmania
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- 作者：Daniela M. Resende ; Brá ; ulia C. Caetano ; Mí ; riam S. Dutra ; Marcus L.O. Penido ; Christiane F. Abrantes ; Rodrigo M. Verly ; Jarbas M. Resende ; Dorila Piló ; -Veloso ; Simone Aparecida Rezende ; O
- 关键词：Leishmaniais ; Recombinant vaccine ; Epitope mapping
- 刊名：Vaccine
- 出版年：2008
- 出版时间：18 August 2008
- 年：2008
- 卷：26
- 期：35
- 页码：4585-4593
- 全文大小：935 K
文摘
A2 was identified as an amastigote virulence factor of Leishmania (Leishmania) donovani and as a candidate antigen for vaccine development against visceral leishmaniasis. Here, predicted hydrophilic, class I and II MHC-binding synthetic peptides were used to define epitopes recognized by A2-specific antibodies, CD8+ T and CD4+ T cells, respectively. Immunization of BALB/c mice with adenovirus expressing A2 (AdA2) resulted in low antibody response, contrasting with high levels of IFN-γ producing CD4+ T and CD8+ T cells specific for A2. Further, A2-specific CD8+ T cells from immunized mice were capable of lysing sensitized target cells in vivo. Finally, we demonstrated an association of A2-specific T cell responses and reduced parasitism in both liver and spleen from mice immunized with AdA2 and challenged with L. (L.) chagasi.