LC/MS/MS analysis of vesnarinone and its principal metabolites in plasma and urine

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• 作者：Tata ; Prasad N.V. ; Schorno ; Karl S. ; Cowart ; T. Douglas ; Bramer ; Steven L.
• 关键词：LC/MS/MS ; Vesnarinone ; Metabolites ; Parent ion ; Product ion
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：1999
• 出版时间：January, 1999
• 年：1999
• 卷：18
• 期：6
• 页码：1037-1045
• 全文大小：121 K

文摘

An LC/MS/MS assay was developed and successfully used to quantitate vesnarinone and its principal metabolites (OPC-8230, OPC-18136, and OPC-18137) in human plasma and urine. Samples were pre-treated with liquid–solid extraction followed by simultaneous monitoring of primary and daughter ions which were used for the identification and quantitation of the analytes on LC/MS/MS. This assay offers advantages of specificity, speed and greater sensitivity over the previously developed HPLC-UV assay. The lower limit of quantitation is 500 ng ml⁻¹ for vesnarinone and 20 ng ml⁻¹ for OPC-8230, OPC-18137, and OPC-18136 in plasma. Methodology is similar for the estimation of these analytes in urine with the lower limit of quantitation being 500 ng ml⁻¹ for vesnarinone and 100 ng ml⁻¹ for each metabolite. Ascorbic acid was added to stabilize the analytes from degradation. This LC/MS/MS method was developed to overcome many practical problems associated with the HPLC method. The LC/MS/MS method offers the flexibility of analyzing additional metabolites and changing the linearity range to accommodate the differences in linear range (200–10 000 ng ml⁻¹ for vesnarinone and 20–1000 for metabolites) for the analytes.