Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17¦Á-ethinylestradiol

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• 作者：Ikumi Hirakawa^a ; ^b ; ^c ; ¹ ; Shinichi Miyagawa^b ; ¹ ; Yoshinao Katsu^d ; Yoshihiro Kagami^c ; Norihisa Tatarazako^e ; Tohru Kobayashi^f ; Teruhiko Kusano^c ; Takeshi Mizutani^b ; Yukiko Ogino^b ; Takashi Takeuchi^a ; ^g ; Yasuhiko Ohta^a ; ^g ; Taisen Iguchi^b ; ^{taisen@nibb.ac.jp}
• 关键词：Medaka ; 17¦Á-Ethinylestradiol ; Testis&ndash ; ova ; Zona pellucida genes ; Microarray
• 刊名：Chemosphere
• 出版年：2012
• 出版时间：May, 2012
• 年：2012
• 卷：87
• 期：7
• 页码：668-674
• 全文大小：859 K

文摘

The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100 ng L^? of 17¦Á-ethinylestradiol (EE2) for 3-5 weeks, and 800 ng estradiol benzoate (EB) for 3 weeks (experiment I), and a measured concentration of 20 ng L^? EE2 for 1-6 weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4 weeks of exposure to 100 ng L^? EE2, 3 weeks of exposure to 800 ng EB, and 6 weeks of exposure to 20 ng L^? EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.