Synthesis and sorption properties of a polyampholyte

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• 作者：Marí ; a Florencia Leal Denis ; Romina Raquel Carballo ; Alej ; ro Javier Spiaggi ; Paula Cecilia Dabas ; Viviana Campo Dall&#x2019 ; Orto ; Juan Manuel Lá ; zaro Martí ; nez ; Graciela Yol ; a Buldain
• 关键词：Polyampholyte ; 2-Methylimidazole ; Methacrylic acid ; Ethylene glycol diglycidyl ether ; Bovine serum albumin
• 刊名：Reactive and Functional Polymers
• 出版年：2008
• 出版时间：January 2008
• 年：2008
• 卷：68
• 期：1
• 页码：169-181
• 全文大小：443 K

文摘

A novel material that combines mechanical resistance with ampholyte character was easily obtained by the reaction of methacrylic acid, 2-methylimidazole and ethylene glycol diglycidyl ether. The structure was studied by solid-state ¹³C NMR and FT IR spectroscopy, scanning electron microscopy (SEM) and potentiometric titration.

The interaction of this new material with bovine serum albumin (BSA) as a model protein was explored. The protein bound electrostatically to the basic form of the polyampholyte at low ionic strength even when both species had negative net charge. The BSA–polyampholyte complex was evidenced by FT IR and SEM.

The adsorption properties were optimal, attributed to the combination of basic and acid groups in the same network, with a maximum loading capacity of 0.7307 g g⁻¹ and a K_d: 5.70 × 10⁻⁶ evaluated by the Langmuir model.

The release of BSA was controlled by dissociation kinetics of the complex rather than by diffusion, and the total desorbed amount depended on the electrolyte content of the medium.