Knock down of the myostatin gene by RNA interference increased body weight in chicken
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- 作者：T.K. Bhattacharya ; bhattacharyatk@gmail.com ; R. Shukla ; R.N. Chatterjee ; K. Dushyanth
- 关键词：Body weight ; Chicken ; Myostatin ; shRNA
- 刊名：Journal of Biotechnology
- 出版年：2017
- 出版时间：10 January 2017
- 年：2017
- 卷：241
- 期：Complete
- 页码：61-68
- 全文大小：1014 K
- 卷排序：241
文摘
Myostatin is a negative regulator of muscular growth in poultry and other animals. Of several approaches, knocking down the negative regulator is an important aspect to augment muscular growth in chicken. Knock down of myostatin gene has been performed by shRNA acting against the expression of gene in animals. The hatching percentage in embryo manipulation and sperm mediated method of knock down was 58.0 and 41.5%, respectively. The shRNA in knock down chicken enhanced body weight at 6 weeks by 26.9%. The dressing percentage and serum biochemical parameters such as SGPT and alkaline phosphatase differed significantly (P < 0.05) between knock down and control birds. It is concluded that knocking down of the myostatin gene successfully augmented growth in chicken.