Auxin-induced hydrogen sulfide generation is involved in lateral root formation in tomato

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• 作者：Tao Fang ; Zeyu Cao ; Jiale Li ; Wenbiao Shen ; Liqin Huang
• 关键词：Auxin ; Cell cycle regulatory gene ; l-Cysteine desulfhydrase ; Hydrogen sulfide ; Lateral root formation ; Sodium hydrosulfide ; Solanum lycopersicum L
• 刊名：Plant Physiology and Biochemistry
• 出版年：March, 2014
• 年：2014
• 卷：76
• 期：Complete
• 页码：44-51
• 全文大小：1516 K

文摘

Similar to auxin, hydrogen sulfide (H₂S), mainly produced by l-cysteine desulfhydrase (DES; EC 4.4.1.1) in plants, could induce lateral root formation. The objective of this study was to test whether H₂S is also involved in auxin-induced lateral root development in tomato (Solanum lycopersicum L.) seedlings. We observed that auxin depletion-induced down-regulation of transcripts of SlDES1, decreased DES activity and endogenous H₂S contents, and the inhibition of lateral root formation were rescued by sodium hydrosulfide (NaHS, an H₂S donor). However, No additive effects were observed when naphthalene acetic acid (NAA) was co-treated with NaHS (lower than 10聽mM) in the induction of lateral root formation. Subsequent work revealed that a treatment with NAA or NaHS could simultaneously induce transcripts of SlDES1, DES activity and endogenous H₂S contents, and thereafter the stimulation of lateral root formation. It was further confirmed that H₂S or HS^{鈭?/sup>, not the other sulfur-containing components derived from NaHS, was attributed to the stimulative action. The inhibition of lateral root formation and decreased of H₂S metabolism caused by an H₂S scavenger hypotaurine (HT) were reversed by NaHS, but not NAA. Molecular evidence revealed that both NaHS- or NAA-induced modulation of some cell cycle regulatory genes, including the up-regulation of SlCDKA;1, SlCYCA2;1, together with simultaneous down-regulation of SlKRP2, were differentially reversed by HT pretreatment. To summarize, above results clearly suggested that H₂S might, at least partially, act as a downstream component of auxin signaling to trigger lateral root formation.}