Construction and evaluation of an exopolysaccharide-producing engineered bacterial strain by protoplast fusion for microbial enhanced oil recovery

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• 作者：Shanshan Sun ; Yijing Luo ; Siyuan Cao ; Wenhong Li ; Zhongzhi Zhang ; Lingxi Jiang ; Hanping Dong ; Li Yu ; Wei-Min Wu
• 关键词：Crude oil ; Microbial enhanced oil recovery (MEOR) ; Water-insoluble exopolysaccharide ; Protoplast fusion ; Fusant
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：144
• 期：Complete
• 页码：44-49
• 全文大小：393 K

文摘

Enterobacter cloacae strain JD, which produces water-insoluble biopolymers at optimal temperature of 30 ¡ãC, and a thermophilic Geobacillus strain were used to construct an engineered strain for exopolysaccharide production at high temperatures by protoplast fusion. The obtained fusant strain ZR3 produced exopolysaccharides at up to 45 ¡ãC with optimal growth temperature at 35 ¡ãC. The fusant produced exopolysaccharides of approximately 7.5 g/L or more at pH between 7.0 and 9.0. The feasibility of the enhancement of crude oil recovery with the fusant was tested in a sand-packed column at 40 ¡ãC. The results demonstrated that bioaugmentation of the fusant was promising approach for MEOR. Mass growth of the fusant was confirmed in fermentor tests.