Construction and expression of an antimicrobial peptide scolopin 1 from the centipede venoms of Scolopendra subspinipes mutilans in Escherichia coli using SUMO fusion partner

详细信息    查看全文

• 作者：Huanhuan Hou ; Weili Yan ; Kexing Du ; Yangjing Ye ; Qianqian Cao ; Wenhua Ren
• 关键词：Centipede ; Scolopendra subspinipes mutilans ; Antimicrobial peptide ; AMP-scolopin 1 ; SUMO
• 刊名：Protein Expression and Purification
• 出版年：December, 2013
• 年：2013
• 卷：92
• 期：2
• 页码：230-234
• 全文大小：1112 K

文摘

Antimicrobial peptide scolopin 1 (AMP-scolopin 1) is a small cationic peptide identified from centipede venoms of Scolopendra subspinipes mutilans. It has broad-spectrum activities against bacteria, fungi, and tumor cells, which may possibly be used as an antimicrobial agent. We first report here the application of small ubiquitin-related modifier (SUMO) fusion technology to the expression and purification of cationic antimicrobial peptide AMP-scolopin 1. The fusion protein expressed in a soluble form was purified to a purity of 95% by Ni-IDA chromatography. After the SUMO-scolopin 1 fusion protein was cleaved by the SUMO protease at 30 掳C for 1 h, the cleaved sample was reapplied to a Ni-IDA. The recombinant scolopin1 had similar antimicrobial properties to the synthetic scolopin 1. Thus, we successfully established a system for purifying peptide of centipede, which could be used for further research.