RasIns: Genetically Encoded Intrabodies of Activated Ras Proteins

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• 作者：Mehmet Cetin¹ ; William E. Evenson² ; Garrett G. Gross¹ ; Farzad Jalali-Yazdi³ ; Daniel Krieger² ; Don Arnold¹ ; Terry T. Takahashi² ; Richard W. Roberts¹ ; ² ; ³ ; ⁴ ; ^{richrob@usc.edu}
• 关键词：Raf-RBD ; Raf-kinase Ras Binding Domain ; SPR ; surface plasmon resonance ; HRP ; horseradish peroxidase ; PBS ; Phosphate Buffered Saline ; TBS ; Tris Buffered Saline ; COS cells ; a cell line that is CV-1 in Origin ; and carrys SV40 genetic material
• 刊名：Journal of Molecular Biology
• 出版年：2017
• 出版时间：17 February 2017
• 年：2017
• 卷：429
• 期：4
• 页码：562-573
• 全文大小：1073 K
• 卷排序：429

文摘

We used mRNA display and an e10FnIII scaffold to find state-specific binders of Ras: RasIn1 and RasIn2. Rasin1 and RasIn2 are stable and functional inside the cells. RasIn1 and RasIn2 bind K-Ras, H-Ras, and oncogenic mutants, but not Rap1B or Arf1 homologs. RasIn2 has similar affinity for Ras as the Ras-binding domain of Raf1, the canonical binding partner of Ras.