A microtiter plate-based β-lactam binding assay for inhibitors of high-molecular-mass penicillin-binding proteins

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• 作者：Miglena Stefanova ; Sudheer Bobba ; William G. Gutheil
• 刊名：Analytical Biochemistry
• 出版年：2010
• 出版时间：1 January 2010
• 年：2010
• 卷：396
• 期：1
• 页码：164-166
• 全文大小：220 K

文摘

High-molecular-mass penicillin-binding proteins (HMM PBPs) are essential for bacterial cell wall biosynthesis and are the lethal targets of β-lactam antibiotics. When purified, HMM PBPs give undetectable or weak enzyme activity. This has impeded efforts to develop assays for HMM PBPs and to develop new inhibitors for HMM PBPs as HMM PBP targeted antibacterial agents. However, even when purified, HMM PBPs retain their ability to bind β-lactams. Here we describe a fluorescently detected microtiter plate-based assay for inhibitor binding to HMM PBPs based on competition with biotin–ampicillin conjugate (BIO–AMP) binding.