Protective role of functionalized single walled carbon nanotubes enhance ex vivo expansion of hematopoietic stem and progenitor cells in human umbilical cord blood
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- 作者：Sudipto Bari ; Pat Pak Yan Chu ; Andrea Lim ; Xiubo Fan ; Florence Pik Hoon Gay ; Ralph Milford Bunte ; Tony Kiat Hon Lim ; Shang Li ; Gigi Ngar Chee Chiu ; William Ying Khee Hwang
- 关键词：CCD ; Charge-Couple Device ; CD ; Cluster of Differentiation ; CFU ; Colony Forming Units ; CFU-GEMM ; Colony Forming Unit-Granulocyte ; Erythorcyte ; Macrophage ; Monocyte ; CFU-GM ; Colony Forming Unit-Granulocyte ; Macrophage ; CLP ; Common Lymphoid Progenitors ; CMP ; Common Myeloid Progenitors ; CNT ; Carbon Nanotubes ; DMSO ; Dimethyl Sulfoxide ; DPBS ; Dulbecco's Phosphate Buffered Saline ; f-SWCNT ; Functionalized Single-Walled Carbon Nanotubes ; f-SWCNT-COOH ; Carboxylic Acid Functionalized Single-Walled Carbon N
- 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
- 出版年：November, 2013
- 年：2013
- 卷：9
- 期：8
- 页码：1304-1316
- 全文大小：4284 K
文摘
In this study, carboxylic acid functionalized single walled carbon nanotubes (f-SWCNT-COOH) was shown to support the viability and ex vivo expansion of freeze-thawed, non-enriched hematopoietic stem and progenitor cells (HSPC) in human umbilical cord blood-mononucleated cells (UCB-MNC). Our in vitro experiments showed that f-SWCNT-COOH increased the viability of the CD45+ cells even without cytokine stimulation. It also reduced mitochondrial superoxides and caspase activity in CD45+ cells. f-SWCNT-COOH drastically reduced the proportions of CD45鈭?/sup> cells in the non-enriched UCB-MNC. Phenotypic expression analysis and functional colony forming units (CFU) showed significant ex vivo expansion of HSPC, particularly of CD45+CD34+CD38鈭?/sup> population and granulocyte-macrophage (GM) colonies, in f-SWCNT-COOH augmented cultures supplemented with basal cytokines. In vivo data suggested that f-SWCNT-COOH expanded UCB-MNC could repopulate immunodeficient mice models with minimal acute or sub-acute symptoms of graft-versus-host disease (GVHD) and f-SWCNT-COOH dependent toxicity.
From the Clinical Editor
In this paper a novel method is presented by using single wall functionalized carbon nanotubes to enhance viability and ex vivo expansion of freeze-thawed, non-enriched hematopoietic stem and progenitor cells in human umbilical cord blood -mononucleated cells. Detailed data is presented about enhanced viability, including improved repopulation of immunodeficient mice models with minimal acute or sub-acute symptoms of graft-versus-host disease.