Optogenetic Inhibitor of the Transcription Factor CREB
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- 作者：Ahmed M. Ali1 ; 4 ; Jakeb M. Reis1 ; Yan Xia2 ; Asim J. Rashid3 ; Valentina Mercaldo3 ; Brandon J. Walters3 ; Katherine E. Brechun1 ; Vitali Borisenko1 ; Sheena A. Josselyn3 ; John Karanicolas2 ; G. Andrew Woolley1 ; awoolley@chem.utoronto.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：photo-control ; photoactive yellow protein ; PYP ; optogenetics ; photoisomerization ; genetically encoded ; CREB ; endogenous ; transcription factor ; dominant negative
- 刊名：Chemistry & Biology
- 出版年：2015
- 出版时间：19 November 2015
- 年：2015
- 卷：22
- 期：11
- 页码：1531-1539
- 全文大小：2207 K
文摘
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Specific dominant negative (DN) inhibitors exist for many transcription factors
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Fusing DNs to optogenetic domains permits optical control of native transcription
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Opto-DN-CREB was created by fusing a DN for CREB to the optogenetic domain PYP
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Optical control of CREB function in vitro, in HEK cells, and in cultured neurons