Experimental reproduction of wobbly possum disease (WPD).
Three types of infectious inocula used, including the purified nidovirus isolate.
WPD diagnosed in 11/12 infected possums based on clinical signs and histopathology.
High levels of viral RNA in tissues of WPD-affected possums, but not in controls.
Our data support aetiological involvement of novel possum nidovirus in WPD.