Anti-diabetic effect of Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) fruit acetone fraction in a type 2 diabetes model of rats

详细信息    查看全文

• 作者：Aminu Mohammed^a ; ^c ; Neil Anthony Koorbanally^b ; Md. Shahidul Islam^a ; ^{islamd@ukzn.ac.za" class="auth_mail" title="E-mail the corresponding author} ; ^{sislam1974@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AI ; atherogenic index ; ALP ; alkaline phosphatase ; ALT ; alanine transaminase ; AST ; aspartate transaminase ; BRU ; Biomedical Resource Unit ; CK-MB ; creatine kinase ; CRI ; coronary artery risk index ; DBC ; diabetic control ; DMF ; diabetic metformin ; DXAH ; diabetic Xylopia aethiopica high dose ; DXAL ; diabetic Xylopia aethiopica low dose ; ELISA ; enzyme-linked immunosorbent assay ; FBG ; fasting blood glucose ; GC&ndash ; MS ; Gas chromatography&ndash ; mass spectroscopy ; HOMA-β ; homeostatic model assessment-β cel
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：2 March 2016
• 年：2016
• 卷：180
• 期：Complete
• 页码：131-139
• 全文大小：1501 K

文摘

In traditional medicine from West Africa, the fruit decoction of m>Xylopia aethiopicam> (Dunal) A. Rich. is widely used for the treatment of diabetes mellitus (DM) either alone or in combination with other plants. The present study is designed to investigate the anti-diabetic effects of m>X. aethiopicam> acetone fraction (XAAF) from fruit ethanolic extract in a type 2 diabetes (T2D) model of rats.

Materials and methods

T2D was induced in rats by feeding a 10% fructose solution m>ad libitumm> for 2 weeks followed by a single intraperitoneal injection of streptozotocin (40 mg/kg body weight) and the animals were orally treated with 150 or 300 mg/kg body weight (bw) of the XAAF once daily for four weeks.

Results

After 4 weeks study period, diabetic untreated animals (DBC) exhibited significantly higher serum glucose, serum fructosamine, LDH, CK-MB, serum lipids, liver glycogen, insulin resistance (HOMA-IR), AI, CRI and lower serum insulin, β-cell function (HOMA-β) and glucose tolerance ability compared to the normal animals. Histopathological examination of their pancreas revealed corresponding pathological changes in the islets and β-cells. These alterations were reverted to near-normal after the treatment of XAAF at 150 (DXAL) and 300 (DXAH) mg/kg bw with the effects being more pronounced in the DXAH group compared to the DXAL group. Moreover, the effects in the animals of DXAH group were comparable to the diabetic metformin (DMF) treated animals. In addition, no significant alterations were observed in non-diabetic animals treated with 300 mg/kg bw of XAAF (NXAH).

Conclusion

The results of our study suggest that XAAF treatment showed excellent anti-diabetic effects in a T2D model of rats.