Separating chemotherapy-related developmental neurotoxicity from cytotoxicity in monolayer and neurosphere cultures of human fetal brain cells
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- 作者：Delyan P. Ivanova ; delyan.ivanov@nottingham.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Abdal-jabbar Al-Rubaib ; mbxaa10@nottingham.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Anna M. Grabowskaa ; 1 ; anna.grabowska@nottingham.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Margaret K. Prattenb ; 1 ; margaret.pratten@nottingham.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Neural stem cell ; Preclinical screening model ; In vitro neurotoxicity assay ; Neuronal differentiation ; Topoisomerase drugs ; Repurposing chemotherapeutics
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：37
- 期：Complete
- 页码：88-96
- 全文大小：1154 K
文摘
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Human brain cells used to compare neurotoxicity to cytotoxicity for anticancer drugs.
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Neurons stained for βIII-tubulin, astrocytes for GFAP, all nuclei (DAPI) in monolayer
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Topoisomerase II drugs were 5–10 times less toxic to neurons compared to all cells.
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Levels of neuronal and glial marker proteins in 3D confirmed lower neurotoxicity.
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