Bioactive lignan constituents from the twigs of Sambucus williamsii

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• 作者：Won Se Suh^a ; Lalita Subedi^b ; ^c ; Sun Yeou Kim^b ; ^c ; Sang Un Choi^d ; Kang Ro Lee^a ; ^{krlee@skku.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sambucus williamsii ; Lignan ; Anti-inflammation ; NGF regulation ; Cytotoxicity
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2016
• 出版时间：15 April 2016
• 年：2016
• 卷：26
• 期：8
• 页码：1877-1880
• 全文大小：411 K

文摘

As part of our ongoing search for bioactive constituents of natural Korean medicinal plants, three new lignan derivatives, sambucasinol A–C (1–3), together with 7 known compounds (4–10) were isolated from the twigs of m>Sambucus williamsiim>. The structures of these new compounds were determined by a combination of 1D and 2D NMR spectroscopic data analysis, as well as circular dichroism (CD) spectroscopy studies. Here, we evaluated the anti-inflammatory effects of 1–10 in lipopolysaccharide (LPS)-stimulated murine microglia BV-2 cells. Compounds 1–3 exhibited significant inhibitory effects on nitric oxide production in LPS-activated BV-2 cells, with IC₅₀ values of 6.82, 7.04, and 14.70 μM, respectively. Additionally, we evaluated the effects of compounds 1–10 on NGF induction in a C6 rat glioma cell line. Compounds 1–3 upregulated NGF secretion to 183.95 ± 2.63%, 153.99 ± 5.15%, and 155.96 ± 5.15%, respectively, without any significant cell toxicity. Moreover, all isolates were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and XF498 cell lines. Compounds 1–3 showed consistent cytotoxicity against the four human cell lines, with IC₅₀ values in the range of 11.07–19.62 μM.