Expression, purification and identification of a thermolysin-like protease, neutral protease I, from Aspergillus oryzae with the Pichia pastoris expression system
详细信息 查看全文
- 作者：Xiaojian Ma 910062852@qq.com" class="auth_mail" title="E-mail the corresponding author ; Yunyun Liu liuyunyun0823@126.com" class="auth_mail" title="E-mail the corresponding author ; Qingqing Li 1343811610@qq.com" class="auth_mail" title="E-mail the corresponding author ; Lu Liu 781793179@qq.com" class="auth_mail" title="E-mail the corresponding author ; Li Yi liyi@hubu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Lixin Ma malixing@hubu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Chao Zhai ; chaozhai2016@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Neutral protease I ; Pichia pastoris ; Thermolysin-like protease
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：52-59
- 全文大小：1045 K
文摘
- •
The recombiant neutral protease was expressed in Pichia pastoris at a high level under high-density fermentation.
- •
The gene of recombiant neutral protease was modified based on the codon usage bias of P. Pastoris.
- •
Low concentrations of zinc, calcium and magnesium ions stimulated the recombiant neutral protease enzyme activity.
- •
All of the evidence indicated that the recombiant neutral protease is a thermolysin-like peptidase.