One-pot preparation of uricase-poly(thiophene-3-boronic acid)-Pt_nano composites for high-performance amperometric biosensing of uric acid

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• 作者：Yi HuangAuthor Vitae ; Lijuan BuAuthor Vitae ; Wen WangAuthor Vitae ; Xiaoli QinAuthor Vitae ; Zou LiAuthor Vitae ; Zhao HuangAuthor Vitae ; Yingchun FuAuthor Vitae ; Xiaoli SuAuthor Vitae ; Qingji Xie ; ^{xieqj@hunnu.edu.cn}Author Vitae ; Shouzhuo YaoAuthor Vitae
• 关键词：Uric acid ; Uricase ; Boronic acid-glycoprotein binding ; Polymeric nanocomposites ; Amperometric biosensing
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：177
• 期：Complete
• 页码：116-123
• 全文大小：1260 K

文摘

Chemical oxidation of glycoprotein-bound thiophene-3-boronic acid (TBA) monomer by relatively mild oxidant chloroplatinate in neutral aqueous solution containing uricase yielded uricase-PTBA-Pt_nano bionanocomposite with high enzymatic specific activity on a platinized (Pt_plate) Au electrode, which was then covered by an outer-layer chitosan (CS) film to fabricate a CS/uricase-PTBA-Pt_nano/Pt_plate/Au electrode. This electrode exhibited a linear amperometric response to uric acid concentration from 5 ¦ÌM to 1.2 mM with a sensitivity of 134 ¦ÌA mM^?1 cm^?2, a limit of detection (S/N = 3) of 1 ¦ÌM, and excellent operation/storage stability, which also worked well in serum samples.