A high-throughput flow injection inductively coupled plasma mass spectrometry method for quantification of oligonucleotides
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- 作者：Qiang Tu ; qiang_tu@merck.com" class="auth_mail" title="E-mail the corresponding author ; Erin N. Guidry ; Fanyu Meng ; Tiebang Wang ; Xiaoyi Gong
- 关键词：Oligonucleotide ; High throughput analysis ; Pharmaceutical ; Inductively coupled plasma-mass spectrometry ; Flow injection analysis
- 刊名：Microchemical Journal
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：124
- 期：Complete
- 页码：668-674
- 全文大小：755 K
文摘
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An HPLC-ICP-MS system in flow injection mode for fast and accurate quantification of phosphorus content in oligonucleotides
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Minimal sample consumption (down to 1 μL) overcomes a major drawback of large sample consumption by conventional methods.
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Isobaric interferences at 31P+ are avoided by monitoring 31P16O+ without the addition of oxygen.
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A comparison of FI-ICP-MS and UV for the determination of synthetic small interfering RNA passenger strands