A biosensor prepared by co-entrapment of a glucose oxidase and a carbon nanotube within an electrochemically deposited redox polymer multilayer

详细信息    查看全文

• 作者：Qiang Gao ; ^a ; ^{gaoqiang@snnu.edu.cn"" rel=""nofollow} ; Yanyan Guo^a ; Jing Liu^a ; Xiaqing Yuan^a ; Honglan Qi^a ; Chengxiao Zhang ; ^a [Author vitae]
• 关键词：Glucose biosensor ; Layer-by-layer electrodeposition ; Single-walled carbon nanotubes ; Redox polymer ; Screen-printed carbon electrode
• 刊名：Bioelectrochemistry
• 出版年：2011
• 出版时间：June 2011
• 年：2011
• 卷：81
• 期：2
• 页码：109-113
• 全文大小：920 K

文摘

A glucose biosensor based on a nanocomposite made by layer-by-layer electrodeposition of the redox polymer into a multilayer containing glucose oxidase (GOx) and single-walled carbon nanotubes (SWCNT) on a screen-printed carbon electrode (SPCE) surface was developed. The objectives of the electrodeposition of redox polymer are to stabilize further the multilayer using a coordinative cross-linked redox polymer and to wire the GOx. The electrochemistry of the layer-by-layer assembly of the GOx/SWCNT/redox polymer nanocomposite was followed by cyclic voltammetry. The resultant biosensor provided stable and reproducible electrocatalytic responses to glucose, and the electrocatalytic current for glucose oxidation was enhanced with an increase in the number of layers. The biosensor displayed a linear range from 0.5 to 6.0 mM, a sensitivity of 16.4 μA/(mM cm²), and a response time of about 5 s. It shows no response to 0.05 mM of ascorbic acid, 0.32 mM of uric acid and 0.20 mM of acetaminophen using a Nafion membrane covering the nanocomposite-modified electrode surface.