Enhancement of phenazine-1-carboxylic acid production using batch and fed-batch culture of gacA inactivated Pseudomonas sp. M18G
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- 作者：Yaqian Li ; Haixia Jiang ; Xilin Du ; Xianqing Huang ; Xuehong Zhang ; Yuhong Xu ; Yuquan Xu
- 关键词：Pseudomonas sp. M18G ; Phenazine-1-carboxylic acid ; Batch culture ; Fed-batch culture ; DO-stat feeding
- 刊名：Bioresource Technology
- 出版年：2010
- 出版时间：May 2010
- 年：2010
- 卷：101
- 期：10
- 页码：3649-3656
- 全文大小：463 K
文摘
The enhancement of phenazine-1-carboxylic acid (PCA) production was investigated in a 10 l fermentor using a gacA chromosomally inactivated mutant Pseudomonas sp. M18G combined with DO-stat feeing strategy. In batch culture, the optimal growth conditions with a two-stage agitation and aeration at 1.72vvm improved PCA production to 1987 mg/l at 48 h fermentation. A mathematical kinetic model consisting of cell growth, high PCA production, and substrate consumption was developed to simulate the batch culture process. Further increases in PCA production were achieved in a DO-stat fed-batch system which maintained a constant DO of 20 % by a two-pulse glucose feeding strategy. With a total 6.6 g/l glucose feeding, the maximum PCA production of 2597 mg/l was obtained at 72 h fermentation, resulting in a 44.5 % increase in PCA production and 10-fold greater cell numbers compared to the batch culture. The combination strategies might pave a new way for the phenazine derivative commercial production using various pseudomonads.