文摘
10?3 DNAzyme (DrzBC) could block the expression of HBV e-gene with application limit of dependence on exogenous delivery. Stearic acid grafted chitosan oligosaccharide (CSSA) could self-aggregate to form micelles in aqueous medium and bind with DrzBC by electrostatic interaction. Compared with Lipofectamine?2000, the CSSA micelles showed much lower cytotoxicity (412.5 ¦Ìg/mL) and advantaged target in subcellular organelles-cytoplasm. Both including DrzBC of 1 ¦Ìmol/L, Lipofectamine?2000/DrzBC complex showed maximum inhibition rate (IR) on HBeAg expression of 46.53 ¡À 2.00 % at 48 h, and then decreased rapidly, while CSSA/DrzBC complex showed maximum IR of 82.51 ¡À 1.28 % at 72 h, and hold on IR above 70 % until 96 h. Moreover, within a concentration range of 0.1?.0 ¦Ìmol/L, and equally incubating for 48 h, the IR of Lipofectamine?2000/DrzBC complex was from 23.20 ¡À 1.61 % to 66.27 ¡À 1.96 % , while the IR of CSSA/DrzBC complex increased from 40.23 ¡À 3.28 % to 80.95 ¡À 1.69 % . CSSA/DrzBC complex is a promising effective system for inhibiting HBeAg expression.