Sustained delivery of dbcAMP by poly(propylene carbonate) micron fibers promotes axonal regenerative sprouting and functional recovery after spinal cord hemisection
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- 作者：Tongliang Xia ; Shilei Ni ; Xingang Li ; Jun Yao ; Hongxu Qi ; Xiaoyong Fan ; Jiangang Wang
- 关键词：PPC ; poly(propylene carbonate) ; dbcAMP ; dibutyryl cyclic adenosine monophosphate ; SCI ; spinal cord injury ; CO2 ; carbon dioxide ; SEM ; scanning electron microscopy ; GAP-43 ; growth-associated protein 43 ; NF-200 ; neurofilament-200 ; GFAP ; glial fibrillary acidic protein ; BBB ; Basso ; Beattie ; and Bresnahan ; chABC ; chondroitin sulfate ABC ; DMEM ; Dulbecco's modified Eagle's medium ; DAB ; 3 ; 3&prime ; -diaminobenzidine ; ELISA ; enzyme-linked immunosorbent assay
- 刊名：Brain Research
- 出版年：13 November, 2013
- 年：2013
- 卷：1538
- 期：Complete
- 页码：41-50
- 全文大小：12224 K
文摘
This study describes the use of poly(propylene carbonate) (PPC) electrospun fibers as vehicle for the sustained delivery of dibutyryl cyclic adenosine monophosphate (dbcAMP) to the hemisected spinal cord. The dbcAMP and PPC were uniformly mixed with acetonitrile; then, electrospinning was used to generate micron fibers. The release of dbcAMP was assessed by ELISA in vitro. Our results showed that the encapsulation of dbcAMP in the fibers led to stable and prolonged release in vitro. The PPC micron fibers containing dbcAMP and the PPC micron fibers without dbcAMP were then implanted into the hemisected thoracic spinal cord, followed by testing of the functional recovery and immunohistochemistry. Compared with the control group, sustained delivery of dbcAMP promoted axonal regenerative sprouting and functional recovery and reduced glial scar formation, and the PPC micron fibers without dbcAMP did not have these effects. Our findings demonstrated the feasibility of using PPC electrospun fibers containing dbcAMP for spinal cord injury. The approach described here also will provide a platform for the potential delivery of other axon-growth-promoting or scar-inhibiting agents.