SDF-1¦Á induces angiogenesis after traumatic brain injury

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• 作者：Shenghui Li^a ; ¹ ; ^{lishenghui2000@gmail.com} ; Ming Wei^b ; ¹ ; ^{neurowei@yahoo.com.cn} ; Ziwei Zhou^a ; ^{1985zhouziwei@163.com} ; Bin Wang^a ; ^{tianjinwangb@163.com} ; Xinliang Zhao^c ; ^{zxl6869@126.com} ; Jianning Zhang^a ; ^{jianningzhang@hotmail.com}
• 关键词：TBI ; traumatic brain injury ; SDF-1 ; stromal cell derived factor-1 ; qRT-PCR ; quantitative real time polymerase chain reaction ; mNSS ; modified neurological severity score ; MVM ; Morris water maze ; MVD ; microvessel density
• 刊名：Brain Research
• 出版年：2012
• 出版时间：20 March, 2012
• 年：2012
• 卷：1444
• 期：Complete
• 页码：76-86
• 全文大小：993 K

文摘

This study aimed to investigate the effects of SDF-1¦Á on brain angiogenesis and neurological functional recovery in rats after traumatic brain injury (TBI) and the potentially involved mechanisms. Youth male Wistar rats were injured via lateral fluid percussion injury and then randomly divided into one of 3 groups: I. vehicle treated group; II. SDF-1¦Á neutralizing antibody treated group and III. rhSDF-1¦Á treated group. rhSDF-1¦Á and its neutralizing antibody or normal saline were administered to the brain penumbra via stereotactic injection 30 min after TBI. Modified neurological severity score (mNSS) and Morris water maze (MWM) test were used to assess the neurologic functional recovery (n = 6/group). 14 days after injury, animals were euthanized and brain tissues were collected for quantitative real time polymerase chain reaction (qRT-PCR) (n = 6/group) and immunohistochemistry (n = 6/group) analysis. mNSS and MWM test indicated distinct amelioration of neurological disability in rhSDF-1¦Á group(P < 0.05). Microvessel density (MVD) of rhSDF-1¦Á treated animals was remarkably increased around the injured area. On the contrary, MVD of the SDF-1¦Á antibody administrated group was significantly decreased compared to that of vehicle treated animals (P < 0.05). The mNSS and MVD had significant negative correlation as tested by Spearman rank correlation coefficient. Immunofluorescence staining showed that CD34 and CXCR4 co-expressed on microvessels. The rhSDF-1¦Á treated animals had greater, contrarily, the SDF-1¦Á antibody treated animals had lesser number of double positive microvessels compared to that of vehicle treated animals. The mRNA expression of CD34 and CXCR4 was obviously elevated in the rhSDF-1¦Á administration group, conversely, declined in SDF-1¦Á antibody treated animals around the injured area compared with that of the vehicle treatment group (P < 0.05). These data indicated that SDF-1¦Á could induce angiogenesis after TBI, potentially via SDF-1/CXCR4 axis.