Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans

详细信息    查看全文

• 作者：Qian Feng^aAuthor Vitae ; Zhuo-Ying Hu^b ; Xue-Qing Liu^a ; Xue Zhang^a ; Xi Lan^a ; Yan-Qing Geng^a ; Xue-Mei Chen^a ; Jun-Lin He^a ; Ying-Xiong Wang^a ; Yu-Bin Ding^a ; ^{dingyb@gmail.com}
• 关键词：embryo implantation ; decidualization ; SLP-2 ; stromal cells
• 刊名：Reproductive BioMedicine Online
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：34
• 期：2
• 页码：191-202
• 全文大小：3450 K
• 卷排序：34

文摘

The molecular mechanisms underlying endometrial stromal cell proliferation and differentiation (decidualization) are still not fully understood. This study revealed that increased Slp-2 expression is a significant factor modulating endometrial stromal cell proliferation and decidualization in both mice and humans. Our results showed a significant difference in the mRNA and protein levels between the implantation site and inter-implantation site on day 5 and day 6 of pregnancy in mice (all P < 0.05). Strong Slp-2 immunostaining was mainly localized within the decidual zone of mice through the post-implantation period. Mice with artificially induced deciduoma showed significantly higher expression of Slp-2 compared with uninduced controls (P < 0.005). Human stromal cells in the middle and late-secretory phases demonstrated significantly (all P < 0.05) upregulated SLP-2, compared with cells in the proliferative phase and early secretory phases. Further analyses of the SLP-2 gene knocked down revealed a significant (P < 0.005) repression of both the decidualization marker gene's expression (decidual/trophoblast prolactin-related protein in mice, insulin-like growth factor binding protein and prolactin in human) and the cell proliferation in in vitro-induced decidualized primary endometrial stromal cells in mice and humans.