Crystal structure and biochemical features of dye-decolorizing peroxidase YfeX from Escherichia coli O157 Asp¹⁴³ and Arg²³² play divergent roles toward different substrates

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• 作者：Xiuhua Liu^a ; ¹ ; ^{liuxiuhua_2004@163.com} ; Zenglin Yuan^b ; ¹ ; Jiaxu Wang^a ; Yaqi Cui^a ; Shuang Liu^a ; Yinliang Ma^a ; Lichuan Gu^b ; Sujuan Xu^b ; ^{xu_sujuan@sdu.edu.cn}
• 关键词：YfeX ; Dye-decolorizing peroxidase ; Crystal structure ; Reactive blue 19 ; ABTS ; Guaiacol
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：26 February 2017
• 年：2017
• 卷：484
• 期：1
• 页码：40-44
• 全文大小：923 K
• 卷排序：484

文摘

We determined the crystal structure of YfeX bound to heme at 2.09 Å resolution. The conserved Asp143 and Arg232 play different roles toward divergent substrates. The H2O2 access tunnel spans from the protein molecular surface to the heme distal region.