Stimulation of lymphocyte proliferation by oyster glycogen sulfated at C-6 position

详细信息    查看全文

• 作者：Jingfeng Yang^a ; ^b ; Beiwei Zhu^a ; ^b ; ^{zhubeiwei@163.com} ; Jie Zheng^c ; Liming Sun^b ; Dayong Zhou^b ; Xiuping Dong^b ; Chenxu Yu^d
• 关键词：Oyster ; Glycogen ; Polysaccharide ; Sulfation ; Structure&ndash ; activity correlation ; Lymphocyte proliferation
• 刊名：Carbohydrate Polymers
• 出版年：2013
• 出版时间：15 April, 2013
• 年：2013
• 卷：94
• 期：1
• 页码：301-308
• 全文大小：845 K

文摘

In this study, glycogen was extracted from oyster Ostrea talienwhanensis Crosse and used as a model to investigate the structure-activity correlation of polysaccharides. Purified oyster glycogen was characterized by methylation analysis, nuclear magnetic resonance (NMR) spectroscopy and infrared spectroscopy (IR). The oyster glycogen was subsequently sulfated by chlorosulfonic acid-pyridine method, and a C-6 substituted species (SOG) was identified to be the primary sulfated oyster glycogen species by ¹³C NMR spectroscopy. The molecular weight and sulfate content of the SOG was determined to be 3.2 ¡Á 10⁴ g/mol and 33.6 % , respectively. Another sulfated oyster glycogen species (SOG1) with C-2 and C-3 substitution was also identified at a lesser amount in the final product. SOG exhibited a much stronger stimulation effect to splenic lymphocyte proliferation than SOG1 in vitro, indicating that the position of sulfate substitution is a major determining factor on the efficacy of sulfated glycogens to stimulate lymphocyte proliferation.