BMP-2 and bFGF in an irradiated bone model

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• 作者：Ingo N.G. Springer ; Peter Niehoff ; Yahya A&#xe7 ; il ; Matthias Marget ; Arne Lange ; Patrick H. Warnke ; Helge Pielenz ; J. Camilo Rold&#xe1 ; n ; Jö ; rg Wiltfang
• 关键词：bone-regeneration ; osteoradionekrosis ; BMP-2 ; bFGF ; craniectomy
• 刊名：Journal of Cranio-Maxillofacial Surgery
• 出版年：2008
• 出版时间：June 2008
• 年：2008
• 卷：36
• 期：4
• 页码：210-217
• 全文大小：2281 K

文摘

Summary

Introduction

Basic fibroblast growth factor (bFGF) is considered to enhance angiogenesis and to support bone formation in the presence of vital bone cells. Bone morphogenetic protein-2 (rhBMP-2) is known to induce bone formation. The aim of this study was to analyze the effect of bFGF and rhBMP-2 in the irradiated mandible.

Material and methods

The right mandibles of 24 rats were irradiated with a single dose of 20 Gy at a high-dose-rate (HDR) after loading machine (bio effective equivalent dose to ca. 45 × 2 Gy). After 12 weeks 100 μg rhBMP-2 (n = 6 animals, group 1), 100 μg bFGF (n = 6 animals, group 2) and 100 μg rhBMP-2 plus 100 μg bFGF (n = 6 animals, group 3) were injected along the right mandible (left mandible: no irradiation, no growth factor). Another 6 animals (group 4) remained untreated after the irradiation. After another 7 weeks the specimens were examined by non-decalcified histology.

Results

Bone apposition of the experimental versus control sides was not statistically significantly different when one of the growth factors was applied alone (rhBMP-2: p = 0.917; bFGF: p = 0.345). Average bone apposition was significantly decreased on the experimental sides of group 3 (rhBMP-2 + bFGF: p = 0.046) and group 4 (p = 0.008). Average bone densities were unaffected in all settings (for all p > 0.1).

Conclusions

The application of bFGF and the application of rhBMP-2 alone did result in predictable bone generation in the irradiated mandible with the bone apposition being equal to that of the non-irradiated side. The application of both growth factors together or none at all after irradiation results in significantly reduced bone apposition.